Proteinase K Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A20453
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| Article Name: | Proteinase K Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A20453 |
| Supplier Catalog Number: | A20453 |
| Alternative Catalog Number: | ABB-A20453-20UL,ABB-A20453-100UL,ABB-A20453-500UL,ABB-A20453-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Proteinase K is an endopeptidase belonging to the subtilisin group of serine proteases. Proteinase K was isolated from the mold (Parengyodontium album or previously known as Tritirachium album). Proteinase K has five cysteines. Four of the cysteine residues form two disulfide bonds (34-124 and 179-248, respectively) and one lies below one of the catalytic triads. Proteinase K often binds two calcium ions required for full enzymatic activity. In molecular biology laboratories, proteinase K is useful during preparation of DNA or RNA samples by degrading and inactivating proteins. |
| Clonality: | Polyclonal |
| UniProt: | P06873 |
| Purity: | Affinity purification |
| Target: | Proteinase K |
| Antibody Type: | Primary Antibody |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Fungi. Shipping: Ice Bag |
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Western blot analysis of recombinant Proteinase K Protein using Proteinase K Rabbit pAb (A20453) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: . |
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