eEF2 Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A2068
Article Name: eEF2 Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A2068
Supplier Catalog Number: A2068
Alternative Catalog Number: ABB-A2068-20UL,ABB-A2068-100UL,ABB-A2068-1000UL,ABB-A2068-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: EF2, EF-2, EEF-2, SCA26, eEF2
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
Clonality: Polyclonal
Molecular Weight: 95kDa
NCBI: 1938
UniProt: P13639
Purity: Affinity purification
Sequence: MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGAGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVED
Target: EEF2
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Translation Control,Protein phosphorylation,Endocrine Metabolism,AMPK Signaling Pathway. Shipping: Ice Bag
Western blot analysis of various lysates using eEF2 Rabbit pAb (A2068) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.