[KO Validated] PHD2/EGLN1 Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A21252
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| Article Name: | [KO Validated] PHD2/EGLN1 Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A21252 |
| Supplier Catalog Number: | A21252 |
| Alternative Catalog Number: | ABB-A21252-100UL,ABB-A21252-20UL,ABB-A21252-1000UL,ABB-A21252-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | HPH2, PHD2, SM20, ECYT3, HALAH, HPH-2, HIFPH2, ZMYND6, C1orf12, HIF-PH2, N1 |
| The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). |
| Application Dilute: | WB,1:2000 - 1:20000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,Cancer,Invasion and Metastasis,Endocrine Metabolism,Cardiovascular,Angiogenesis,Hypoxia. Shipping: Ice Bag |
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Western blot analysis of lysates from wild type (WT) and PHD2/EGLN1 knockout (KO) 293T cells, using [KO Validated] PHD2/EGLN1 Rabbit mAb (A21252) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 8s. |
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