AKT1S1/PRAS40 Rabbit pAb, Unconjugated, Polyclonal
Catalog Number:
ABB-A21686
- Images (1)
| Article Name: | AKT1S1/PRAS40 Rabbit pAb, Unconjugated, Polyclonal |
| Biozol Catalog Number: | ABB-A21686 |
| Supplier Catalog Number: | A21686 |
| Alternative Catalog Number: | ABB-A21686-20UL,ABB-A21686-100UL,ABB-A21686-500UL,ABB-A21686-1000UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | Lobe, PRAS40, AKT1S1/PRAS40 |
| AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]). |
| Application Dilute: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,Translation Control,Regulation of eIF4 and p70 S6 Kinase,Protein phosphorylation,Signal Transduction,Kinase,PI3K-Akt Signaling Pathway,mTOR Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Autophagy,Cell Cycle,Endocrine Metabolism,Insulin Receptor Signaling Pathway,Immunology Inflammation,T Cell Receptor Signaling Pathway,Neuroscience. Shipping: Ice Bag |
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Western blot analysis of lysates from PC-3 cells usingAKT1S1/PRAS40 Rabbit pAb (A21686) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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