SPHK1 Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A22093
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| Article Name: | SPHK1 Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A22093 |
| Supplier Catalog Number: | A22093 |
| Alternative Catalog Number: | ABB-A22093-100UL,ABB-A22093-20UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | SPHK, SPHK1 |
| The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Application Dilute: | WB,1:2000 - 1:10000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Cancer,Signal Transduction,G protein signaling,G-Protein-Coupled ReceptorsGPCR,Kinase,Cell Biology Developmental Biology,Apoptosis,Endocrine Metabolism,Lipid Metabolism,Cardiovascular,Angiogenesis. Shipping: Ice Bag |
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Western blot analysis of various lysates using SPHK1 Rabbit mAb (A22093) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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