Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:
Unconjugated
Alternative Names:
Ly-2, Ly-B, Ly-35, Lyt-2, CD8A
Enables identical protein binding activity. Acts upstream of or within several processes, including cytotoxic T cell differentiation, defense response to virus, and positive regulation of calcium-mediated signaling. Located in external side of plasma membrane. Is expressed in several structures, including endocrine gland, exocrine gland, genitourinary system, gut, and hemolymphoid system gland. Orthologous to human CD8A (CD8a molecule).
WB,1:500 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:
Cross-Reactivity: Mouse. Shipping: Ice Bag
Western blot analysis of various lysates, using CD8A Rabbit pAb (A22114) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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