Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:
Unconjugated
Alternative Names:
Il-4, BSF-1, IL4
Enables cytokine activity. Involved in several processes, including innate immune response in mucosa, negative regulation of white fat cell proliferation, and regulation of gene expression. Acts upstream of or within several processes, including T-helper cell differentiation, positive regulation of macromolecule metabolic process, and regulation of leukocyte activation. Located in external side of plasma membrane and extracellular space. Is expressed in several structures, including brain, colon, hemolymphoid system, liver, and placenta. Used to study Sjogrens syndrome, atopic dermatitis, and type 1 diabetes mellitus. Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple), autoimmune disease (multiple), hepatitis B, hepatitis C, and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).
WB,1:1000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:
Cross-Reactivity: Mouse. ResearchArea: Immunology,Innate Immunity,Cytokines,Interleukins,Immunology,Adaptive Immunity,Regulatory T Cells,Neuroscience,Processes. Shipping: Ice Bag
Western blot analysis of various lysates, using IL4 Rabbit mAb (A22284) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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