NEDD8 Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A22568
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| Article Name: | NEDD8 Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A22568 |
| Supplier Catalog Number: | A22568 |
| Alternative Catalog Number: | ABB-A22568-20UL,ABB-A22568-100UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | NEDD-8, NEDD8 |
| Enables ubiquitin protein ligase binding activity. Acts upstream of or within protein neddylation. Located in cytosol and nucleoplasm. Biomarker of Parkinsons disease and malignant astrocytoma. |
| Application Dilute: | WB,1:1000 - 1:6000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cell Biology Developmental Biology,Ubiquitin. Shipping: Ice Bag |
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Western blot analysis of various lysates using NEDD8 Rabbit mAb (A22568) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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