HCoV-229E Spike S2 Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A22604
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| Article Name: | HCoV-229E Spike S2 Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A22604 |
| Supplier Catalog Number: | A22604 |
| Alternative Catalog Number: | ABB-A22604-20UL,ABB-A22604-100UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| S1 region attaches the virion to the cell membrane by interacting with host ANPEP/aminopeptidase N, initiating the infection. Binding to the receptor probably induces conformational changes in the S glycoprotein unmasking the fusion peptide of S2 region and activating membranes fusion. S2 region belongs to the class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. |
| Application Dilute: | WB,1:1000 - 1:3000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: HCoV-229E. Shipping: Ice Bag |
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Western blot analysis of lysates from Human coronavirus (HCoV-229E) Spike Protein (S1+S2 ECD His Tag) using HCoV-229E Spike S2 Rabbit mAb (A22604) at 1:3000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 100ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45 s. |
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