KAT2B/PCAF Rabbit mAb, Unconjugated, Monoclonal

Article Name:	KAT2B/PCAF Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A22719
Supplier Catalog Number:	A22719
Alternative Catalog Number:	ABB-A22719-100UL,ABB-A22719-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC-P, IP, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	CAF, PCAF, P/CAF, KAT2B/PCAF

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

Clonality:	Monoclonal
Molecular Weight:	93 kDa
NCBI:	8850
UniProt:	Q92831
Purity:	Affinity purification
Target:	KAT2B

Application Dilute:	WB,1:500 - 1:2000\|IP,0.5µg-4µg antibody for 400µg-600µg extracts of whole cells\|IHC-P,1:200 - 1:2000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Epigenetic writers and erasers of core Histones,Nuclear Receptor Signaling,Signal Transduction,Cell Biology Developmental Biology,Cell Cycle,Cell cycle inhibitors,Cell Cycle Control-G2 M DNA Damage Checkpoint,Immunology Inflammation,NF-kB Signaling Pathway. Shipping: Ice Bag

	Western blot analysis of various lysates using KAT2B/PCAF Rabbit mAb (A22719) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry analysis of paraffin-embeddedRat colon tissue usingKAT2B/PCAF Rabbit mAb(A22719) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat kidney tissue usingKAT2B/PCAF Rabbit mAb(A22719) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse colon tissue usingKAT2B/PCAF Rabbit mAb(A22719) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse kidney tissue usingKAT2B/PCAF Rabbit mAb(A22719) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman pancreas tissue usingKAT2B/PCAF Rabbit mAb(A22719) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman thyroid tissue usingKAT2B/PCAF Rabbit mAb(A22719) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunoprecipitation of KAT2B/PCAF from 600 µg extracts of Hep G2 cells was performed using 2 µg of KAT2B/PCAF Rabbit mAb (A22719). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using KAT2B/PCAF Rabbit mAb (A22719) at a dilution of 1:1000.