Myeloperoxidase (MPO) Rabbit mAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A22900
- Images (8)
| Article Name: | Myeloperoxidase (MPO) Rabbit mAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A22900 |
| Supplier Catalog Number: | A22900 |
| Alternative Catalog Number: | ABB-A22900-500UL,ABB-A22900-100UL,ABB-A22900-1000UL,ABB-A22900-20UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | MPO, myeloperoxidase, Myeloperoxidase (MPO) |
| Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. |
| Application Dilute: | WB,1:3000 - 1:10000|IF/ICC,1:50 - 1:200|IF-P,1:50 - 1:200|IHC-P,1:5000 - 1:20000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Cell Biology Developmental Biology,Apoptosis,Endocrine Metabolism,Immunology Inflammation,Cell Intrinsic Innate Immunity Signaling Pathway,Cardiovascular,Blood. Shipping: Ice Bag |
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Western blot analysis of lysates from HL-60 cells using Myeloperoxidase (MPO) Rabbit mAb (A22900) at 1:10000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC):HeLa Exposure time: 30s. |
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Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Myeloperoxidase (MPO) Rabbit mAb (A22900) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Myeloperoxidase (MPO) Rabbit mAb (A22900) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Myeloperoxidase (MPO) Rabbit mAb (A22900) at a dilution of 1:6000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Confocal imaging of paraffin-embedded Rat spleen tissue using Myeloperoxidase (MPO) Rabbit mAb (A22900, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
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Confocal imaging of paraffin-embedded Mouse spleen tissue using Myeloperoxidase (MPO) Rabbit mAb (A22900, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
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Confocal imaging of paraffin-embedded Human colon tissue using Myeloperoxidase (MPO) Rabbit mAb (A22900, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
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Confocal imaging of HL-60 cells using Myeloperoxidase (MPO) Rabbit mAb (A22900, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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