Hsp90beta Rabbit mAb, Unconjugated, Monoclonal

Article Name:	Hsp90beta Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A23489
Supplier Catalog Number:	A23489
Alternative Catalog Number:	ABB-A23489-100UL,ABB-A23489-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	HSP84, HSPC2, HSPCB, D6S182, HSP90B, Hsp90beta

This gene encodes a member of the heat shock protein 90 family, these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.

Clonality:	Monoclonal
Molecular Weight:	83kDa
NCBI:	3326
UniProt:	P08238
Purity:	Affinity purification
Target:	HSP90AB1

Application Dilute:	WB,1:2000 - 1:20000\|IHC-P,1:100 - 1:500\|IF/ICC,1:100 - 1:500\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat,Rice. ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Nuclear Receptor Signaling,Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Cytoskeleton,Actins,Endocrine Metabolism,Mitochondrial metabolism. Shipping: Ice Bag

	Western blot analysis of various lysates, using Hsp90beta Rabbit mAb (A23489) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry analysis of paraffin-embeddedHuman breast cancer tissue usingHsp90beta Rabbit mAb(A23489) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse testis tissue usingHsp90beta Rabbit mAb(A23489) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse colon tissue usingHsp90beta Rabbit mAb(A23489) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman colon carcinoma tissue usingHsp90beta Rabbit mAb(A23489) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat kidney tissue usingHsp90beta Rabbit mAb(A23489) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat testis tissue usingHsp90beta Rabbit mAb(A23489) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Confocal imaging of 293T cells using Hsp90beta Rabbit mAb (A23489, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.