Alpha Fodrin Rabbit mAb, Unconjugated, Monoclonal

Article Name:	Alpha Fodrin Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A2418
Supplier Catalog Number:	A2418
Alternative Catalog Number:	ABB-A2418-20UL,ABB-A2418-100UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	DEE5, NEAS, EIEE5, SPTA2, Alpha Fodrin

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.

Clonality:	Monoclonal
Molecular Weight:	285kDa
NCBI:	6709
UniProt:	Q13813
Purity:	Affinity purification
Target:	SPTAN1

Application Dilute:	WB,1:500 - 1:1000\|IHC-P,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Apoptosis,Cytoskeleton,Microfilaments,Death Receptor Signaling Pathway. Shipping: Ice Bag

	Western blot analysis of various lysates using Alpha Fodrin Rabbit mAb (A2418) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Western blot analysis of lysates from HeLa cells, using Alpha Fodrin Rabbit mAb (A2418) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry analysis of paraffin-embeddedRat colon tissue usingAlpha Fodrin Rabbit mAb(A2418) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman thyroid cancer tissue usingAlpha Fodrin Rabbit mAb(A2418) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman liver cancer tissue usingAlpha Fodrin Rabbit mAb(A2418) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman colon carcinoma tissue usingAlpha Fodrin Rabbit mAb(A2418) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse colon tissue usingAlpha Fodrin Rabbit mAb(A2418) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat kidney tissue usingAlpha Fodrin Rabbit mAb(A2418) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.