NAGS Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A24553
Article Name: NAGS Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A24553
Supplier Catalog Number: A24553
Alternative Catalog Number: ABB-A24553-100UL,ABB-A24553-500UL,ABB-A24553-20UL,ABB-A24553-1000UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: NAGS, AGAS, ARGA, N-acetylglutamate synthase
The N-acetylglutamate synthase gene encodes a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAG is a cofactor of carbamyl phosphate synthetase I (CPSI), the first enzyme of the urea cycle in mammals. This gene may regulate ureagenesis by altering NAG availability and, thereby, CPSI activity. Deficiencies in N-acetylglutamate synthase have been associated with hyperammonemia.
Clonality: Polyclonal
Molecular Weight: 58kDa
NCBI: 162417
UniProt: Q8N159
Purity: Affinity purification
Target: NAGS
Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse. ResearchArea: Cancer,Signal Transduction,Endocrine & Metabolism,Mitochondrial metabolism,Mitochondrial markers,Amino acid metabolism. Shipping: Ice Bag
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with NAGS Rabbit pAb, using NAGS Rabbit pAb (A24553) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.