CRBN Rabbit mAb, Unconjugated, Monoclonal

Article Name:	CRBN Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A24651
Supplier Catalog Number:	A24651
Alternative Catalog Number:	ABB-A24651-20UL,ABB-A24651-100UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	MRT2, MRT2A, CRBN

This gene encodes a protein related to the Lon protease protein family. In rodents and other mammals this gene product is found in the cytoplasm localized with a calcium channel membrane protein, and is thought to play a role in brain development. Mutations in this gene are associated with autosomal recessive nonsyndromic cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene.

Clonality:	Monoclonal
Molecular Weight:	51kDa
NCBI:	51185
UniProt:	Q96SW2
Purity:	Affinity purification
Target:	CRBN

Application Dilute:	WB,1:1000 - 1:4000\|IHC-P,1:200 - 1:2000\|IF/ICC,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Neuroscience,Neurodegenerative Diseases. Shipping: Ice Bag

	Western blot analysis of various lysates, using CRBN Rabbit mAb (A24651) at 1:1500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20 s.
	Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CRBN Rabbit mAb (A24651) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse colon using CRBN Rabbit mAb (A24651) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse spleen using CRBN Rabbit mAb (A24651) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat colon using CRBN Rabbit mAb (A24651) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Confocal imaging of 293T cells using CRBN Rabbit mAb (A24651,dilution 1:200)(Red). DAPI was used for nuclear staining (blue). Objective: 100x.
	Confocal imaging of NIH/3T3 cells using CRBN Rabbit mAb (A24651,dilution 1:200)(Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
	Confocal imaging of PC-3 cells using CRBN Rabbit mAb (A24651,dilution 1:200)(Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.