CD45 Rabbit PolymAb, Unconjugated, Monoclonal

Article Name:	CD45 Rabbit PolymAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A24802PM
Supplier Catalog Number:	A24802PM
Alternative Catalog Number:	ABB-A24802PM-100UL,ABB-A24802PM-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human, Mouse
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180, IMD105

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.

Clonality:	Monoclonal
Molecular Weight:	180-240 kDa
NCBI:	5788
UniProt:	P08575
Purity:	Affinity purification
Target:	CD45

Application Dilute:	WB,1:500 - 1:1000\|IF-F,1:200 - 1:800\|IF-P,1:200 - 1:800\|IHC-P,1:1000 - 1:4000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse. ResearchArea: Signal Transduction,Kinase,Tyrosine kinases,Cell Biology Developmental Biology,Apoptosis,Immunology Inflammation,CDs,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,Neuroscience, Cell Type Marker,Stem Cells,Hematopoietic Progenitors. Shipping: Ice Bag

	Western blot analysis of various lysates, using CD45 Rabbit PolymAb (A24802PM) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control(NC):MCF-7,C2C12 Exposure time: 15s.
	Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD45 Rabbit PolymAb (A24802PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human liver tissue using CD45 Rabbit PolymAb (A24802PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse intestine tissue using CD45 Rabbit PolymAb (A24802PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using CD45 Rabbit PolymAb (A24802PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using CD45 Rabbit PolymAb (A24802PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Confocal imaging of Mouse spleen using CD45 Rabbit PolymAb (A24802PM,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
	Confocal imaging of paraffin-embedded Human spleen tissue using CD45 Rabbit PolymAb (A24802PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.