CD324/E-Cadherin Rabbit PolymAb, Unconjugated, Monoclonal

Article Name:	CD324/E-Cadherin Rabbit PolymAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A24874PM
Supplier Catalog Number:	A24874PM
Alternative Catalog Number:	ABB-A24874PM-20UL,ABB-A24874PM-100UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Mouse
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	Um, UVO, Ecad, ARC-1, E-cad, L-CAM

This gene encodes E-cadherin, a calcium-dependent cell adhesion molecule that functions in the establishment and maintenance of epithelial cell morphology during embryongenesis and adulthood. The encoded preproprotein undergoes proteolytic processing to generate a mature protein. Targeted mutations disrupting binding of calcium to the encoded protein in mice cause death in utero due to failed blastocyst and trophectoderm formation. This gene is located adjacent to a related cadherin gene on chromosome 8.

Clonality:	Monoclonal
Molecular Weight:	98kDa
NCBI:	12550
UniProt:	P09803
Purity:	Affinity purification
Target:	Cdh1

Application Dilute:	WB,1:1000 - 1:4000\|IF-F,1:200 - 1:400\|IF-P,1:200 - 1:800\|IHC-P,1:700 - 1:7000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Mouse,Rat. ResearchArea: Cancer,Invasion and Metastasis,Signal Transduction,ErbB-HER Signaling Pathway,Cell Biology & Developmental Biology,Apoptosis,Cell Cycle,Centrosome,Cell Adhesion,Cadherins,Tight Junctions,Cytoskeleton,Wnt/beta-Catenin Signaling Pathway,Immunology & Inflammati. Shipping: Ice Bag

	Western blot analysis of lysates from Rat liver using CD324/E-Cadherin Rabbit PolymAb (A24874PM) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using CD324/E-Cadherin Rabbit PolymAb (A24874PM) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using CD324/E-Cadherin Rabbit PolymAb (A24874PM) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using CD324/E-Cadherin Rabbit PolymAb (A24874PM) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using CD324/E-Cadherin Rabbit PolymAb (A24874PM) at a dilution of 1:7000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Confocal imaging of paraffin-embedded Mouse colon tissue using CD324/E-Cadherin Rabbit PolymAb (A24874PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of paraffin-embedded Rat colon tissue using CD324/E-Cadherin Rabbit PolymAb (A24874PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of paraffin-embedded Rat liver tissue using CD324/E-Cadherin Rabbit PolymAb (A24874PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.