HSPB8/HSP22 Rabbit mAb, Unconjugated, Monoclonal

Article Name:	HSPB8/HSP22 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A25396
Supplier Catalog Number:	A25396
Alternative Catalog Number:	ABB-A25396-100UL,ABB-A25396-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	H11, HMN2, CMT2L, DHMN2, E2IG1, HMN2A, HSP22

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

Clonality:	Monoclonal
Molecular Weight:	22kDa
NCBI:	26353
UniProt:	Q9UJY1
Purity:	Affinity purification
Target:	HSPB8

Application Dilute:	WB,1:5000 - 1:30000\|IF/ICC,1:200 - 1:800\|IF-P,1:200 - 1:800\|IHC-P,1:200 - 1:800\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat,Monkey. ResearchArea: Signal Transduction,Neuroscience,Neurodegenerative Diseases. Shipping: Ice Bag

	Western blot analysis of lysates from COS-7 cells usingHSPB8/HSP22 Rabbit mAb (A25396) at1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
	Western blot analysis of various lysates usingHSPB8/HSP22 Rabbit mAb (A25396) at1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC):HL-60. Exposure time:10s.
	Immunohistochemistry analysis of paraffin-embeddedRat heart tissue usingHSPB8/HSP22 Rabbit mAb(A25396) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse heart tissue usingHSPB8/HSP22 Rabbit mAb(A25396) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman skeletal muscle tissue usingHSPB8/HSP22 Rabbit mAb(A25396) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Confocal imaging of U-2 OS cells usingHSPB8/HSP22 Rabbit mAb (A25396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging ofparaffin-embedded Mouse heart tissue usingHSPB8/HSP22 Rabbit mAb (A25396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
	Confocal imaging of RD cells usingHSPB8/HSP22 Rabbit mAb (A25396, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.