Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:
PerCP/Cy5.5
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells, by non-specific interactions with cellular proteins, carbohydrates, and lipids, or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen.An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Clonality:
Monoclonal
Concentration:
FC
Purity:
Affinity purification
Target:
IgG isotype control
Application Dilute:
FC,5 µl per 10 6 cells in 100 µl volume
Application Notes:
Shipping: Ice Bag
Flow cytometry: 1X10 6 293T cells (negative control,left)and Jurkat cells (right) were surface-stained with PerCP/Cyanine5.5 Rabbit anti-Human CD45 mAb (A25622,5 µl/Test,orange line) or PerCP/Cyanine5.5 Rabbit IgG isotype control (A25620,5 µl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).
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