CD71/Transferrin Receptor Rabbit mAb, Unconjugated, Monoclonal

Article Name:	CD71/Transferrin Receptor Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A25900
Supplier Catalog Number:	A25900
Alternative Catalog Number:	ABB-A25900-20UL,ABB-A25900-1000UL,ABB-A25900-500UL,ABB-A25900-100UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, IP, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	T9, TR, TFR, p90, CD71, TFR1, TRFR, IMD46

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified.

Clonality:	Monoclonal
Molecular Weight:	85 kDa
NCBI:	7037
UniProt:	P02786
Purity:	Affinity purification
Target:	TFRC

Application Dilute:	WB,1:1000 - 1:4000\|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells\|IF/ICC,1:50 - 1:200\|IF-P,1:50 - 1:200\|IHC-P,1:500 - 1:2000\|ELISA,Recommended starting concentration is 1 µg/mL.Please optimize the concentration based on your specific assay
Application Notes:	Cross-Reactivity: Human,Mouse. ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Cancer,Endocrine Metabolism,Immunology Inflammation,CDs,Stem Cells,Hematopoietic Progenitors,Neural Stem Cells,Cardiovascular,Blood,Serum Proteins,Hypoxia. Shipping: Ice Bag

	Western blot analysis of various lysates using CD71/Transferrin Receptor Rabbit mAb (A25900) at 1:20000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5 s.
	Western blot analysis of lysates from RAW 264.7 cells using CD71/Transferrin Receptor Rabbit mAb (A25900) at 1:20000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45 s.
	Confocal imaging of paraffin-embedded Human placenta tissue using CD71/Transferrin Receptor Rabbit mAb (A25900, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of K-562 cells using CD71/Transferrin Receptor Rabbit mAb (A25900, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging of paraffin-embedded Mouse colon tissue using CD71/Transferrin Receptor Rabbit mAb (A25900, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
	Immunoprecipitation of CD71/Transferrin Receptor from 300 µg extracts of K-562 cells was performed using 1 µg of CD71/Transferrin Receptor Rabbit mAb (A25900). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using CD71/Transferrin Receptor Rabbit mAb (A25900) at a dilution of 1:1000.
	Confocal imaging of paraffin-embedded Mouse colon tissue using CD71/Transferrin Receptor Rabbit mAb (A25900, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
	Immunoprecipitation of CD71/Transferrin Receptor from 300 µg extracts of K562 cells was performed using 0.5 µg of CD71/Transferrin Receptor Rabbit mAb (A25900). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using CD71/Transferrin Receptor Rabbit mAb (A25900) at a dilution of 1:1000.