CD36/SR-B3 Rabbit mAb, Unconjugated, Monoclonal

Article Name:	CD36/SR-B3 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A26251
Supplier Catalog Number:	A26251
Alternative Catalog Number:	ABB-A26251-20UL,ABB-A26251-100UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	FAT, GP4, GP3B, GPIV, CHDS7, PASIV, SCARB3, BDPLT10

The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. It binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. It directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and it binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Mutations in this gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants have been found for this gene.

Clonality:	Monoclonal
Molecular Weight:	53kDa
NCBI:	948
UniProt:	P16671
Purity:	Affinity purification
Target:	CD36

Application Dilute:	WB,1:1800-1:6000\|IF-P,1:50 - 1:200\|IHC-P,1:1000 - 1:4000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Endocrine Metabolism,Lipid Metabolism,Immunology Inflammation,CDs,Stem Cells,Hematopoietic Progenitors,Cardiovascular,Blood,Heart,Lipids,Cardiovascular diseases,Heart disease. Shipping: Ice Bag

	Western blot analysis of various lysates using CD36/SR-B3 Rabbit mAb (A26251) at 1:3000 dilutionincubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): C2C12. Exposure time: 45 s.
	Western blot analysis of lysates from 3T3-L1 Day 11 Adipocytes cells using CD36/SR-B3 Rabbit mAb (A26251) at 1:3000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45 s.
	Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using CD36/SR-B3 Rabbit mAb (A26251) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using CD36/SR-B3 Rabbit mAb (A26251) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human colon tissue using CD36/SR-B3 Rabbit mAb (A26251) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human breast tissue using CD36/SR-B3 Rabbit mAb (A26251) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.
	Confocal imaging of paraffin-embedded Mouse spleen tissue using CD36/SR-B3 Rabbit mAb (A26251, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of paraffin-embedded Mouse lung tissue using CD36/SR-B3 Rabbit mAb (A26251, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.