HLA-DR Rabbit PolymAb, Unconjugated, Monoclonal
Catalog Number:
ABB-A26694PM
- Images (8)
| Article Name: | HLA-DR Rabbit PolymAb, Unconjugated, Monoclonal |
| Biozol Catalog Number: | ABB-A26694PM |
| Supplier Catalog Number: | A26694PM |
| Alternative Catalog Number: | ABB-A26694PM-100UL,ABB-A26694PM-1000UL,ABB-A26694PM-500UL,ABB-A26694PM-20UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | HLA-DRA1 |
| HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. This molecule is expressed on the surface of various antigen presenting cells such as B lymphocytes, dendritic cells, and monocytes/macrophages, and plays a central role in the immune system and response by presenting peptides derived from extracellular proteins, in particular, pathogen-derived peptides to T cells. The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. |
| Application Dilute: | WB,1:1000 - 1:4000|IF/ICC,1:200 - 1:800|IF-P,1:200 - 1:800|IHC-P,1:400 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Immunology Inflammation. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using HLA-DR Rabbit PolymAb (A26694PM) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining. |
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Western blot analysis of various lysates using HLA-DR Rabbit PolymAb (A26694PM) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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Immunohistochemistry analysis of paraffin-embedded Human colon tissue using HLA-DR Rabbit PolymAb (A26694PM) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using HLA-DR Rabbit PolymAb (A26694PM) at a dilution of 1:1600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining. |
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Confocal imaging of paraffin-embedded Human tonsil tissue using HLA-DR Rabbit PolymAb (A26694PM, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x. |
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Confocal imaging of Daudi cells using HLA-DR Rabbit PolymAb (A26694PM, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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Confocal imaging of Karpas-299 cells using HLA-DR Rabbit PolymAb (A26694PM, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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Confocal imaging of Raji cells using HLA-DR Rabbit PolymAb (A26694PM, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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