Alpha Internexin Rabbit mAb, Unconjugated, Monoclonal

Article Name:	Alpha Internexin Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A3596
Supplier Catalog Number:	A3596
Alternative Catalog Number:	ABB-A3596-100UL,ABB-A3596-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	NEF5, NF66, NF-66, TXBP-1, Alpha Internexin

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons.

Clonality:	Monoclonal
Molecular Weight:	55kDa
NCBI:	9118
UniProt:	Q16352
Purity:	Affinity purification
Target:	INA

Application Dilute:	WB,1:500 - 1:2000\|IF-P,1:50 - 1:200\|IHC-P,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cell Biology Developmental Biology,Cell Adhesion,Cytoskeleton,Intermediate Filaments,Neuroscience. Shipping: Ice Bag

	Western blot analysis of various lysates using Alpha Internexin Rabbit mAb (A3596) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry analysis of paraffin-embeddedHuman colon tissue usingAlpha Internexin Rabbit mAb(A3596) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat brain tissue usingAlpha Internexin Rabbit mAb(A3596) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman brain tissue usingAlpha Internexin Rabbit mAb(A3596) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman appendix tissue usingAlpha Internexin Rabbit mAb(A3596) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse brain tissue usingAlpha Internexin Rabbit mAb(A3596) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunofluorescence analysis of paraffin-embedded rat brain using Alpha Internexin Rabbit mAb (A3596) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of paraffin-embedded mouse brain using Alpha Internexin Rabbit mAb (A3596) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.