KRT10 Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A7908
Article Name: KRT10 Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A7908
Supplier Catalog Number: A7908
Alternative Catalog Number: ABB-A7908-100UL,ABB-A7908-20UL,ABB-A7908-500UL,ABB-A7908-1000UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: BIE, EHK, IHL, K10, KPP, BCIE, CK10, KRT10
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.
Clonality: Polyclonal
Molecular Weight: 59kDa
NCBI: 3858
UniProt: P13645
Purity: Affinity purification
Sequence: EIDNNIEQISSYKSEITELRRNVQALEIELQSQLALKQSLEASLAETEGRYCVQLSQIQAQISALEEQLQQIRAETECQNTEYQQLLDIKIRLENEIQTYRSLLEGEGSSG
Target: KRT10
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human. ResearchArea: Signal Transduction,Cell Biology Developmental Biology,Cytoskeleton,Intermediate Filaments,Extracellular Matrix,Keratin. Shipping: Ice Bag
Western blot analysis of lysates from MCF7 cells, using KRT10 Rabbit pAb (A7908) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.