SIK3 Rabbit pAb, Unconjugated, Polyclonal

Catalog Number: ABB-A8019
Article Name: SIK3 Rabbit pAb, Unconjugated, Polyclonal
Biozol Catalog Number: ABB-A8019
Supplier Catalog Number: A8019
Alternative Catalog Number: ABB-A8019-100UL,ABB-A8019-20UL,ABB-A8019-1000UL,ABB-A8019-500UL
Manufacturer: ABclonal
Host: Rabbit
Category: Antikörper
Application: ELISA, WB
Species Reactivity: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation: Unconjugated
Alternative Names: L19, QSK, SEMDK, SIK-3, SIK3
Enables ATP binding activity, magnesium ion binding activity, and protein serine/threonine kinase activity. Involved in positive regulation of TORC1 signaling, positive regulation of TORC2 signaling, and protein phosphorylation. Located in nucleoplasm.
Clonality: Polyclonal
Molecular Weight: 145kDa
NCBI: 23387
UniProt: Q9Y2K2
Purity: Affinity purification
Sequence: PARIGYYEIDRTIGKGNFAVVKRATHLVTKAKVAIKIIDKTQLDEENLKKIFREVQIMKMLCHPHIIRLYQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARRKFKQIVTAVYFCHCRNIVHRDLKAENLLLDANLNIKIADFGFSNLFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVLSGKFRIPFFMSTECEHLIRHMLVLDPNKRLSMEQ
Target: SIK3
Antibody Type: Primary Antibody
Application Dilute: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,Kinase,Cell Biology Developmental Biology,Cell Cycle,Centrosome. Shipping: Ice Bag
Western blot analysis of lysates from 293F cells, using SIK3 Rabbit pAb (A8019) at 1:6000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.