Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:
Unconjugated
Alternative Names:
STX13, STX14, STX12
Predicted to enable SNAP receptor activity and SNARE binding activity. Involved in autophagosome assembly, cholesterol efflux, and protein stabilization. Located in several cellular components, including membrane raft, phagocytic vesicle, and phagophore assembly site. Part of SNARE complex. Colocalizes with BLOC-1 complex.
WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:
Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction. Shipping: Ice Bag
Western blot analysis of various lysates using STX12 Rabbit pAb (A8200) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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