IKZF3 Rabbit mAb, Unconjugated, Monoclonal

Article Name:	IKZF3 Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A8614
Supplier Catalog Number:	A8614
Alternative Catalog Number:	ABB-A8614-100UL,ABB-A8614-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, IP, WB
Species Reactivity:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	AIO, IMD84, AIOLOS, ZNFN1A3, IKZF3

This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation. Both Ikaros and Aiolos can participate in chromatin remodeling. Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers. Several alternative transcripts encoding different isoforms have been described, as well as some non-protein coding variants.

Clonality:	Monoclonal
Molecular Weight:	58 kDa
NCBI:	22806
UniProt:	Q9UKT9
Purity:	Affinity purification
Target:	IKZF3

Application Dilute:	WB,1:500 - 1:1000\|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells\|IF-P,1:50 - 1:200\|IHC-P,1:200 - 1:800\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors. Shipping: Ice Bag

	Western blot analysis of various lysates using IKZF3 Rabbit mAb (A8614) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of various lysates using IKZF3 Rabbit mAb (A8614) at 1:1000 dilutionincubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): U-937. Exposure time: 45 s.
	Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using IKZF3 Rabbit mAb (A8614) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using IKZF3 Rabbit mAb (A8614) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using IKZF3 Rabbit mAb (A8614) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunofluorescence analysis of paraffin-embedded rat spleen using IKZF3 Rabbit mAb (A8614) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 300 µg extracts of Raji cells using 3 µg IKZF3 antibody (A8614). Western blot was performed from the immunoprecipitate using IKZF3 antibody (A8614) at a dilution of 1:500.
	Immunoprecipitation analysis of 300 µg extracts of Raji cells using 3 µg IKZF3 antibody (A8614). Western blot was performed from the immunoprecipitate using IKZF3 antibody (A8614) at a dilution of 1:500.