Peroxiredoxin 4 (PRDX4) Rabbit mAb, Unconjugated, Monoclonal

Article Name:	Peroxiredoxin 4 (PRDX4) Rabbit mAb, Unconjugated, Monoclonal
Biozol Catalog Number:	ABB-A9131
Supplier Catalog Number:	A9131
Alternative Catalog Number:	ABB-A9131-100UL,ABB-A9131-20UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	PRX-4, AOE372, AOE37-2, HEL-S-97n, Peroxiredoxin 4 (PRDX4)

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.

Clonality:	Monoclonal
Molecular Weight:	31kDa
NCBI:	10549
UniProt:	Q13162
Purity:	Affinity purification
Target:	PRDX4

Application Dilute:	WB,1:500 - 1:1000\|IHC-P,1:50 - 1:200\|IF/ICC,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Application Notes:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cell Biology Developmental Biology. Shipping: Ice Bag

	Western blot analysis of various lysates using Peroxiredoxin 4 (PRDX4) Rabbit mAb (A9131) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry analysis of paraffin-embeddedHuman thyroid cancer tissue usingPeroxiredoxin 4 (PRDX4) Rabbit mAb(A9131) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman colon carcinoma tissue usingPeroxiredoxin 4 (PRDX4) Rabbit mAb(A9131) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse testis tissue usingPeroxiredoxin 4 (PRDX4) Rabbit mAb(A9131) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman lung cancer tissue usingPeroxiredoxin 4 (PRDX4) Rabbit mAb(A9131) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedRat colon tissue usingPeroxiredoxin 4 (PRDX4) Rabbit mAb(A9131) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman liver tissue usingPeroxiredoxin 4 (PRDX4) Rabbit mAb(A9131) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedHuman cervix cancer tissue usingPeroxiredoxin 4 (PRDX4) Rabbit mAb(A9131) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunofluorescence analysis of NIH/3T3 cells using Peroxiredoxin 4 (PRDX4) Rabbit mAb (A9131) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.