PCNA Mouse mAb, Unconjugated
Catalog Number:
ABB-AC058
- Images (8)
| Article Name: | PCNA Mouse mAb, Unconjugated |
| Biozol Catalog Number: | ABB-AC058 |
| Supplier Catalog Number: | AC058 |
| Alternative Catalog Number: | ABB-AC058-1000UL,ABB-AC058-50UL,ABB-AC058-100UL,ABB-AC058-500UL,ABB-AC058-200UL |
| Manufacturer: | ABclonal |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ELISA, IF, IHC-P, WB |
| Species Reactivity: | Rat |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | PCNAR, Pcna/cyclin |
| Enables estrogen receptor binding activity. Involved in several processes, including estrous cycle, liver regeneration, and response to dexamethasone. Located in nucleus. Biomarker of several diseases, including brain ischemia (multiple), mucositis, obstructive jaundice, osteoporosis, and sciatic neuropathy. Human ortholog(s) of this gene implicated in stomach cancer. Orthologous to human PCNA (proliferating cell nuclear antigen). |
| Application Dilute: | WB,1:1000 - 1:10000|IF/ICC,1:200 - 1:800|IF-P,1:200 - 1:800|IHC-P,1:2000-1:8000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cell Biology Cell Cycle MarkersEpigenetics and Nuclear Signaling DNA methylation Methylated DNA BindingTags & Cell Markers Cell Type Markers ReplicationEpigenetics and Nuclear Signaling DNA / RNA DNA Synthesis OtherEpigenetics and Nuclear Signaling Chromatin Binding Proteins DNA / RNA bindingCancer Cell cycle Cell divisionCancer Tumor biomarkers Tumor antigensIsotype/Loading Controls Loading Controls PCNA. Shipping: Ice Bag |
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Western blot analysis of various lysates using PCNA Mouse mAb (AC058) at 1:5000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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Immunohistochemistry analysis of paraffin-embedded Mouse skin tissue using PCNA Mouse mAb (AC058) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using PCNA Mouse mAb (AC058) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human colon tissue using PCNA Mouse mAb (AC058) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using PCNA Mouse mAb (AC058) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Confocal imaging of HeLa cells using PCNA Mouse mAb (AC058, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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Confocal imaging of NIH/3T3 cells using PCNA Mouse mAb (AC058, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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Confocal imaging of paraffin-embedded Rat small intestine tissue using PCNA Mouse mAb (AC058, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
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