Phospho-Src-Y529 Rabbit mAb, Unconjugated
Catalog Number:
ABB-AP1377
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| Article Name: | Phospho-Src-Y529 Rabbit mAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP1377 |
| Supplier Catalog Number: | AP1377 |
| Alternative Catalog Number: | ABB-AP1377-100UL,ABB-AP1377-20UL,ABB-AP1377-1000UL,ABB-AP1377-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | ASV, SRC1, THC6, c-SRC, p60-Src, Phospho-Src-Y529 |
| This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. |
| Application Dilute: | WB,1:2000 - 1:10000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Cancer,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Tyrosine kinases,ErbB-HER Signaling Pathway,MAPK-Erk Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Cell Adhesion,Cytoskeleton,Microtubules,Actins,Wnt -Catenin Signaling Pathway,Immunology Inflammation,Jak-Stat-IL-6 Receptor Signaling Pathway,Cardiovascular,Angiogenesis. Shipping: Ice Bag |
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Western blot analysis of lysates from A-431 cells, using Phospho-Src-Y529 Rabbit mAb (AP1377) at 1:10000 dilution. A-431 cells were treated with EGF (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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