Phospho-eNOS-S1177 Rabbit mAb, Unconjugated
Catalog Number:
ABB-AP1404
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| Article Name: | Phospho-eNOS-S1177 Rabbit mAb, Unconjugated |
| Biozol Catalog Number: | ABB-AP1404 |
| Supplier Catalog Number: | AP1404 |
| Alternative Catalog Number: | ABB-AP1404-20UL,ABB-AP1404-100UL,ABB-AP1404-1000UL,ABB-AP1404-500UL |
| Manufacturer: | ABclonal |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Conjugation: | Unconjugated |
| Alternative Names: | eNOS, ECNOS, Phospho-eNOS-S1177 |
| Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. |
| Application Dilute: | WB,1:2000 - 1:8000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Application Notes: | Cross-Reactivity: Human. ResearchArea: Protein phosphorylation,Cancer,Signal Transduction,PI3K-Akt Signaling Pathway,Cell Biology Developmental Biology,Endocrine Metabolism,AMPK Signaling Pathway,Neuroscience,Cardiovascular,Angiogenesis,Blood,Hypoxia. Shipping: Ice Bag |
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Western blot analysis of lysates from HUVEC cells using Phospho-eNOS-S1177 Rabbit mAb (AP1404) at 1:6000 dilutionincubated overnight at 4°C. HUVEC cells were treated with Pervanadate (100 µM) at 37°C for 10 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60 s. |
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