Phospho-MAP2K4-S257 Rabbit mAb, Unconjugated

Article Name:	Phospho-MAP2K4-S257 Rabbit mAb, Unconjugated
Biozol Catalog Number:	ABB-AP1491
Supplier Catalog Number:	AP1491
Alternative Catalog Number:	ABB-AP1491-1000UL,ABB-AP1491-500UL,ABB-AP1491-20UL,ABB-AP1491-100UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, IP, WB
Species Reactivity:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	JNKK, MEK4, MKK4, SEK1, SKK1, JNKK1, SERK1, MAPKK4, PRKMK4, SAPKK1, SAPKK-1

This gene encodes a member of the mitogen-activated protein kinase (MAPK) family. Members of this family act as an integration point for multiple biochemical signals and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. They form a three-tiered signaling module composed of MAPKKKs, MAPKKs, and MAPKs. This protein is phosphorylated at serine and threonine residues by MAPKKKs and subsequently phosphorylates downstream MAPK targets at threonine and tyrosine residues. A similar protein in mouse has been reported to play a role in liver organogenesis. A pseudogene of this gene is located on the long arm of chromosome X. Alternative splicing results in multiple transcript variants.

Molecular Weight:	44kDa
NCBI:	6416
UniProt:	P45985
Purity:	Affinity purification
Sequence:	VDS(P)IA
Target:	MAP2K4

Application Dilute:

WB,1:1000 - 1:5000|IHC-P,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,0.5µg-4µg antibody for 200µg-400µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.

Application Notes:

Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Protein phosphorylation,Signal Transduction,G protein signaling,G-Protein-Coupled Receptors Signaling to MAPK Erk,Kinase,Tyrosine kinases,ErbB-HER Signaling Pathway,MAPK-JNK Signaling Pathway,Cell Biology Developmental Biology,Cytoskeleton,Actins,TGF-b-Smad Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,Jak-Stat-IL-6 Receptor Signaling Pathway,Toll-like Receptor Signaling Pathway,Neuroscience,Neurodegenerative Diseases,Dopamine Signaling in Parkinsons Disease. Shipping: Ice Bag

	Immunohistochemistry analysis of paraffin-embedded Human colon tissue, Untreated (left) and lambda phosphatase-treated (right), using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue, Untreated (left) and lambda phosphatase-treated (right), using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Western blot analysis of lysates from C6 cells using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at 1:2600 dilutionincubated overnight at 4°C. C6 cells were treated by UV (90mJ/cm2) at room temperature and recovered for 2 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue, Untreated (left) and lambda phosphatase-treated (right), using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Confocal imaging of HeLa cells (treated with UV) and HeLa cells (untreated) using Phospho-MAP2K4-S257 Rabbit mAb (AP1491, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Immunoprecipitation of Phospho-MAP2K4-S257 from 200 µg extracts of HeLa cells treated by UV (100 mJ/cm2, 4 hours) was performed using 0.5 µg of Phospho-MAP2K4-S257 Rabbit mAb (AP1491). Rabbit IgG isotype control (AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Phospho-MAP2K4-S257 Rabbit mAb (AP1491) at a dilution of 1:1000.