Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb, Unconjugated

Article Name:	Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb, Unconjugated
Biozol Catalog Number:	ABB-AP1551
Supplier Catalog Number:	AP1551
Alternative Catalog Number:	ABB-AP1551-20UL,ABB-AP1551-1000UL,ABB-AP1551-500UL,ABB-AP1551-100UL
Manufacturer:	ABclonal
Host:	Rabbit
Category:	Antikörper
Application:	ELISA, IF, IHC-P, WB
Species Reactivity:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Conjugation:	Unconjugated
Alternative Names:	CDC2, CDC28A, P34CDC2, Phospho-CDK1-T14

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight:	34 kDa
NCBI:	983
UniProt:	P06493
Purity:	Affinity purification
Sequence:	RVYTH
Target:	CDK1

Application Dilute:

WB,1:9500 - 1:19000|IF-P,1:200 - 1:800|IHC-P,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.

Application Notes:

Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Protein phosphorylation,Signal Transduction,G protein signaling,Kinase,Serine threonine kinases,ErbB-HER Signaling Pathway,ATM Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Cell Cycle,Centrosome,Cell Cycle Control-G2 M DNA Damage Checkpoint,Microtubules,Immunology Inflammation,Neuroscience,Neurodegenerative Diseases. Shipping: Ice Bag

	Western blot analysis of lysates from NIH/3T3 cells using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb (AP1551) at 1:19000 dilutionincubated overnight at 4°C. NIH/3T3 cells were treated with lambda-PP mixed solution (2U/ul) at 37°C for 1 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90 s.
	Western blot analysis of lysates from C6 cells using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb (AP1551) at 1:19000 dilutionincubated overnight at 4°C. C6 cells were treated with lambda-PP mixed solution (2U/ul) at 37°C for 1 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180 s.
	Western blot analysis of lysates from A-431 cells using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb (AP1551) at 1:19000 dilutionincubated overnight at 4°C. A-431 cells were treated with EGF (100 ng/mL) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90 s.
	Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb (AP1551) at a dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb (AP1551) at a dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb (AP1551) at a dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb (AP1551) at a dilution of 1:900 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Confocal imaging of paraffin-embedded Mouse testis (untreated) and Mouse testis (treated with lambdaPP) tissue using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb (AP1551, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of paraffin-embedded Rat testis (untreated) and Rat testis (treated with lambdaPP) tissue using Phospho-CDK1-T161+CDK2/CDK3-T160 Rabbit mAb (AP1551, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.