The PMA + Ionomycin T Cell Stimulant for ELISpot is an in vitro reagent commonly used to activate T cells. PMA activates protein kinase C (PKC), triggering downstream signaling pathways that lead to protein expression and T cell activation, while Ionomycin, a calcium ionophore, increases intracellular Ca? levels to further enhance PKC activation. This product is provided as a convenient lyophilized mixture, ready for in vitro use, and is formulated specifically for ELISpot applications.
Samples:
Peripheral blood mononuclear cells (PBMCs) in single-cell suspension, capable of cytokine secretion.
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