Protein A (PA) is a 42 kDa surface protein originally found in the cell wall of the Gram positive bacteria Staphylococcus aureus. It has the specific ability to bind with the constant (Fc) portion of immunoglobulin molecules from various different species. Protein A is encoded by the polymorphic X-region and functions as an immunoglobulin-binding protein, characteristically it has the ability to bind with the -Fc-region- of IgG. Due to its high affinity for Fc region of human IgG, it is extensively used in affinity chromatography process required for the purification of antibodies. However, one of the major disadvantages of this method of purification is co elution or leaching of PA along with the target antibody.
Clonality:
Monoclonal
Clone Designation:
[ABM2A4.1E3]
Purity:
Protein G Chromatography
Form:
Purified
Target:
Protein A
Application Dilute:
Recommended dilutions: WB: 0.1-1 µg/ml, ELISA: 1 µg/ml. However, this need to be optimized based on the research applications.
Figure-1: Western blot analysis of Protein A. Anti-Protein A antibody (Clone: ABM2A4.1E3) was used at 0.1 µg/ml on Recombinant protein.
Figure-2: An indirect ELISA is carried out by coating Protein A in in serial dilution from 05 ng to 0.078ng and using 100 ng of purified ABM2A4.1E3 monoclonal antibody . Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.
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