A mouse isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens.
Clonality:
Monoclonal
Clone Designation:
[231]
Purity:
Affinity Chromatography
Form:
Purified
Antibody Type:
Isotype Control
Application Dilute:
FACS: 0.25-2 µg/10 6 cells
Fig-1: Cell surface flow analysis of Mouse IgG2a isotype control in Ramos cell line using 2 µg/10 6 cells (Clone: 231). Black represents cell only, red represents Mouse IgG2a isotype control. Goat anti-mouse PE conjugate was used as secondary antibody.
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