A mouse isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens.
Clonality:
Monoclonal
Clone Designation:
[231]
Purity:
Affinity Chromatography
Form:
Purified
Antibody Type:
Isotype Control
Application Dilute:
FACS: 0.25-2 ul/10 6 cells
Fig:1- Cell surface FLOW analysis of hCD25 in PHA treated human PBMC using 5 ul /10 6 cells. Green represents PE conjugated mouse IgG2a isotype control (10-102-P), red represents anti-hCD25 antibody (10-4125-P).
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