It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Clonality:
Monoclonal
Clone Designation:
[BRD.3]
Purity:
Affinity Chromatography
Form:
Purified
Target:
Bromodeoxyuridine (BrdU) (Proliferation Marker)
Application Dilute:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with
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