Monoclonal Antibody to HSP27 (Heat Shock Protein 27)(Clone : G3.1), Clone: [G3.1], Mouse
Biozol Catalog Number:
ABI-36-1239
Supplier Catalog Number:
36-1239
Alternative Catalog Number:
ABI-36-1239-100UG
Manufacturer:
Abeomics
Host:
Mouse
Category:
Antikörper
Application:
FACS, IF, IHC, WB
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells.
Alternative Names:
HSPB1||HSP27||HSP28
This MAb reacts specifically with heat shock protein HSP27 in human and monkey tissues and cell lines such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K protein and HSP28, is one of several small heat shock proteins produced by all organisms studied. HSP27 synthesis is induced by elevated temperature, as well as by estrogen in hormone responsive cells. Interestingly, human HSP27 also shares greater than 50% homology with low molecular weight Drosophila HSPs and mammalian alpha -crystalline lens protein. Because of the estrogen responsive nature of HSP27, this protein has been studied extensively in human estrogen responsive tissues such as cervix, endometrium and breast tissue. Therefore HSP27 may be useful in classifying various hormone sensitive tumors.
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM
* VAT and and shipping costs not included. Errors and price changes excepted
