Monoclonal Antibody to Tenascin C (Stromal Marker For Epithelial Malignancy)(Clone : SPM319), Clone: [SPM319], Mouse
Biozol Catalog Number:
ABI-36-1250
Supplier Catalog Number:
36-1250
Alternative Catalog Number:
ABI-36-1250-100UG
Manufacturer:
Abeomics
Host:
Mouse
Category:
Antikörper
Application:
FACS, IF, IHC
Species Reactivity:
Human
Immunogen:
Human breast carcinoma
Alternative Names:
TNC||HXB
In Western blotting, it reacts with two bands of ~MW of 210kDa and 300kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linked hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.
Tenascin C (Stromal Marker For Epithelial Malignancy)
Application Dilute:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min
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