Monoclonal Antibody to MUC1 / EMA / CD227 (Epithelial Marker)(Clone : 139H2), Clone: [139H2], Mouse
Biozol Catalog Number:
ABI-36-1453
Supplier Catalog Number:
36-1453
Alternative Catalog Number:
ABI-36-1453-100UG
Manufacturer:
Abeomics
Host:
Mouse
Category:
Antikörper
Application:
ELISA, FACS, IF, IHC, WB
Species Reactivity:
Human, Mouse
Immunogen:
Human milk-fat globule membranes (HMFGM)
Alternative Names:
MUC1||PUM
This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
ELISA (For coating use Ab at 2-5ug/ml, order Ab without BSA0, ,Flow Cytometry (0.25-0.5ug/million cells), Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formali
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