Monoclonal Antibody to Major Vault Protein (MVP)(Clone : SPM280), Clone: [SPM280], Mouse
Biozol Catalog Number:
ABI-36-1991
Supplier Catalog Number:
36-1991
Alternative Catalog Number:
ABI-36-1991-100UG
Manufacturer:
Abeomics
Host:
Mouse
Category:
Antikörper
Application:
FACS, IF, IHC
Species Reactivity:
Human
Immunogen:
Proteins precipitated from human breast cancer MCF-7 cells
Alternative Names:
MVP||LRP
Recognizes a protein of 104kDa-110kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy.
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95
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