Anti-Nucleolin (Marker of Human Cells) Monoclonal Antibody(Clone: NCL/902)-CF488, Clone: [NCL/902], Mouse
Biozol Catalog Number:
ABI-36-2842
Supplier Catalog Number:
36-2842
Alternative Catalog Number:
ABI-36-2842-0.5AT100MLATUG/ML
Manufacturer:
Abeomics
Host:
Mouse
Category:
Antikörper
Application:
FACS, IF
Species Reactivity:
Human
Immunogen:
Recombinant human NCL protein
Conjugation:
CF488
Alternative Names:
NCL, Nucl, Nucleolin, Protein C23
Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thoµght to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Flow Cytometry (5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood),Immunofluorescence (1:50-1:100),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC
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