Anti-Cytochrome C (Mitochondrial Marker) Monoclonal Antibody(Clone: CYCS/3128R), Clone: [CYCS/3128R], Rabbit
Biozol Catalog Number:
ABI-36-2952-20
Supplier Catalog Number:
36-2952-20
Alternative Catalog Number:
ABI-36-2952-20-20UG
Manufacturer:
Abeomics
Host:
Rabbit
Category:
Antikörper
Application:
FACS, IHC, WB
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant full-length human CYCS protein
Alternative Names:
CYC, CYCS, HCS, THC4
It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thoµght to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This MAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e. cytochrome in the mitochondria with heme attached).
Flow Cytometry (1-2ug/million cells), Western Blot (1-2ug/ml), ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95
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