Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine EGF as the immunizing antigen. This IgG2AK antibody was purified from cell culture by Protein G affinity chromatography.
Form:
A sterile filtered antibody solution was lyophilized from PBS.
Antibody Type:
Monoclonal Antibody
Application Notes:
WesternBlot: To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 0.25-0.50 ng/lane under non-reducing conditions and 10.0-15.0 ng/lane under reducing conditions.. Sandwich: In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 100 pg/ml of recombinant murine EGF when used with BioGems biotinylated antigen affinity purified anti-murine EGF (61-063BT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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