Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hENA-78. Anti-Human ENA-78 specific antibody was purified by affinity chromatography employing immobilized hENA-78 matrix.
Form:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Application Notes:
WesternBlot: To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect hENA-78 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human ENA-78 (60-118BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hENA-78.. Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.0 µg/ml-2.0 µg/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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