Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine IL-10 as the immunizing antigen. This IgG2A antibody was purified from cell culture by Protein G affinity chromatography.
Form:
A sterile filtered antibody solution was lyophilized from PBS.
Antibody Type:
Monoclonal Antibody
Application Notes:
WesternBlot: To detect mIL-10 by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-10 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.. Sandwich: In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 1600 pg/ml of recombinant murine IL-10 when used with BioGems biotinylated antigen affinity purified anti-murine IL-10 (61-010BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine IL-10 (0.5 ng/ml), a concentration of 3.0-5.0 µg/ml of this antibody is required.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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