Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-11. Anti-Human IL-11 specific antibody was purified by affinity chromatography employing immobilized hIL-11 matrix.
Form:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Application Notes:
WesternBlot: To detect hIL-11 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-11 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect hIL-11 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human IL-11 (60-011BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-11.. Immunohistochemistry: This antibody stained human brain stroke (including control cortex and stroke core areas) tissue. The primary antibody was incubated at 2.5 mg/ml overnight at 4°C. This was followed by a fluorophore conjugated secondary antibody. Optimal concentrations and conditions may vary.<br /> <br /> <em><span style=font-size: 10px,>Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm.</em>. Neutralization: To yield one-half maximal inhibition [ND<span style=font-size: 16px,>50] of the biological activity of hIL-11 (1.50 ng/ml), a concentration of 0.02-0.05 µg/ml of this antibody is required.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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