Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine IL-2 as the immunizing antigen. This IgG2Akappa antibody was purified from cell culture by Protein A affinity chromatography.
Form:
A sterile filtered antibody solution was lyophilized from PBS.
Antibody Type:
Monoclonal Antibody
Application Notes:
WesternBlot: To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.. Sandwich: In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 32 pg/ml of recombinant murine IL-2 when used with BioGems biotinylated antigen affinity purified anti-murine IL-2 (61-002BT) as the detection antibody at a concentration of at least 1.0-2.0 µg/ml.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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