Produced in BALB/c mice using highly pure (>98%) recombinant human IL-6 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
Form:
A sterile filtered antibody solution was lyophilized from PBS.
Antibody Type:
Monoclonal Antibody
Application Notes:
WesternBlot: To detect hIL-6 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-6 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.. Sandwich: In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant human IL-6 when used with BioGems biotinylated antigen affinity purified anti-human IL-6 (60-006BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.. Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human cervical squamous cell carcinoma. The recommended concentration is 0.125 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.<br /> <br /> *Additional Immunostaining data available. Please contact Tech Support for information.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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